An experimental study of immune depression to donor origin cells induced by the mixed chimera after sublethal whole body irradiation
- VernacularTitle:受体大鼠非清髓嵌合的建立及对供体细胞反应性的监测
- Author:
Jichen QU
;
Gening JIANG
;
Jiaan DING
;
Wen GAO
;
Zhenya SHEN
- Publication Type:Journal Article
- Keywords:
Chimera;
Immune depression;
Bone marrow mesenchymal stem cells
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immune depression to donor-origin cells induced by the Mixed Chimera after sublethal whole body irradiation.Methods:Recipients in the experiment were Wistar and SD rat,and Wistar rats were selected as the donor.Donor and recipient rats were divided randomly into three groups.Recipient were conditioned with sublethal whole body irradiation (WBI).Group A was infused with bone marrow cells (BMC) of Wistar rats;group B infused with bone marrow mesenchymal stem cells (BM-MSCs)of Wistar rats;and group C with normal saline.Then they were administered cytoxan(CTX) by intraperitoneal injection.The mechanisms for immune depression were explored by performing mixed lymphocyte reaction (MLR).Results:The results showed that donor lymphoid chimeras could be found in the immune depression SD rats and chimerac cells in group A was more than in group B by FCM assay (P
