Effect of VEGF-C Gene Transfection on Expression of VEGF-C in Human Breast Cancer MCF-7 Cell
- VernacularTitle:VEGF-C基因转染对人乳腺癌MCF-7细胞VEGF-C表达的影响
- Author:
Ming SHAN
;
Jianguo ZHANG
;
Baoliang GUO
- Publication Type:Journal Article
- Keywords:
MCF-7 cell line Eukaryotic expression Plasmid Vascular endothelial growth factor-C Gene transfection
- From:
Chinese Journal of Bases and Clinics in General Surgery
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of vascular endothelial growth factor-C (VEGF-C) gene transfection on the expression level of VEGF-C in human breast cancer MCF-7 cell. Methods The constructed VEGF-C gene eukaryotic expression vector was transfected into the human breast cancer MCF-7 cell by using lipofectamine transfection reagents, and the positive cell clones were obtained through G418 selection after transfection. The expressions of VEGF-C mRNA and protein were detected by RT-PCR and Western blot respectively. Results Following the transfection of the VEGF-C recombination plasmid, there were significant differences on the expression levels of VEGF-C mRNA and protein between pcDNA3.1-VEGF-C transfection group and pcDNA3.1 transfection group (12.382?2.183 vs 6.039?1.950, P
