Effect of HMGB1 and expression of TLR-4 in renal injury of SLE
- VernacularTitle:高迁移率族蛋白及其TOLL样受体4在系统性红斑狼疮肾脏损害中的作用
- Author:
Shuxia LIU
;
Huifang GUO
;
Yujun ZHANG
;
Qingjuan LIU
;
Lijuan TANG
;
Huijun DUAN
- Publication Type:Journal Article
- Keywords:
Systemic lupus erythematosus;
Lupus nephritis;
High mobility group protein box;
Toll like receptor 4
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of HMGB1 and TLR-4 in the renal injury of SLE.Methods:The level of HMGB1 in serum from 16 patients suffering from SLE without kidney damage,18 patient with lupus nephritis (LN),and 12 healthy individuals were measured by ELISA.The fresh PBMCs were isolated and the total RNA was extracted.Then the mRNA expression of HMGB1 was amplified by RT-PCR.Flow cytometric analysis(FCM) was performed to study cell surface markers and the expression of TLR-4.Results:RT-PCR and ELISA results showed that the expressions of mRNA and protein were higher in patients with LN than in SLE without kindey damage and healthy people.The expression of TLR-4 in CD14+ monocytes of patients with LN was higher either,while there were no significant changes in CD3+ T cells among LN,SLE and healthy control.Conclusion:PBMCs in patients with LN could synthesize and secrete HMGB1 initiatively,which are correlated with serum HMGB1 level.HMGB1 might play a role in autoimmunity of lupus nephritis partly by activation of monocytes through its binding to TLR-4.
