Stimulation of c-myc protooncogene expression by transforming growth factor a in human ovarian cancer cells.
- Author:
Jeong Youn CHOI
1
;
Young Sook HONG
;
Hae Young PARK
Author Information
1. EWHA WOMANS UNIV,COLL MED, EWHA MED RES CTR, DEPT BIOCHEM, SEOUL 158056, SOUTH KOREA.
- Publication Type:Original Article
- Keywords:
c-myc;
ovarian cancer cells;
TGFalpha
- MeSH:
Cycloheximide;
Dactinomycin;
Half-Life;
Humans*;
Ovarian Neoplasms*;
RNA, Messenger;
Transforming Growth Factor alpha;
Transforming Growth Factors*
- From:Experimental & Molecular Medicine
1997;29(4):203-208
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate whether transforming growth factor alpha (TGF alpha) treatment of human ovarian cancer cells was associated with the induction of c-myc protooncogene, the expression of this gene in NIH:OVCAR-3 cells was examined. TGF alpha induced increase in c-myc mRNA level, with a peak after 1 h of treatment; this stimulation was dose-dependent, with an optimal concentration of 5 ng/ml TGF alpha. Its primary action is probably at the transcription level since the half-life of c-myc mRNA measured in the presence of actinomycin D was not modified by TGF alpha treatment. In addition, TGF alpha stimulation of c-myc mRNA did not require protein synthesis since it was not suppressed by cycloheximide treatment. Antisense phosphorothioate oligonucleotide to c-myc specifically inhibited the TGF alpha-stimulated c-Myc protein expression and growth of NIH:OVCAR-3 cells. Our results indicate that induction of c-myc expression by TGF alpha plays an important role in the growth of NIH:OVCAR-3 cells.
