Expression of VEGF gene mediated by adenovirus in human bone marrow stromal cell
- VernacularTitle:腺病毒介导VEGF基因在人骨髓基质干细胞中的表达
- Author:
Bin CHEN
;
Yanbin SONG
;
Yuhua LI
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cell;
VEGF;
green fluorescent protein;
gene transfection
- From:
Orthopedic Journal of China
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the possibility of stable expression of VEGF~65 in human bone marrow stromal cell.[Method]hBMSc were divided into transfection group, empty vector group and control group, hBMSc were infected with Ad-VEGF165(MOI=20 pfu). The hBMSc proliferation and transfection efficiency were confirmed by fluorescence microscope.The expression of VEGF165 gene of cultured hBMSc transfected with VEGF165 gene was assayed by RT-PCR, ELISA and Western blot.[Result]VEGF165 gene was amplified with RT-PCR.GAPDH gene(internal control) was located at 549 bp in the three groups, VEGF165 gene was located at 500 bp in transfection group but not in the other groups. ELISA assay showed that the expression had significant difference in the transfection group compared with the empty vector group and control group (P
