Study on effects of anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells
- VernacularTitle:抗hDR5单抗对食管癌细胞的凋亡及CDC作用观察
- Author:
Hongying HUANG
;
Yaowu DU
;
Huiling BAI
;
Shulian LI
;
Yuanfang MA
- Publication Type:Journal Article
- Keywords:
Death receptor 5;
Monoclonal antibody;
Cells apoptosis;
CDC effects
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells.Methods:The anti-DR5 monoclonal antibody was prepared by hybridoma technique.Tumoricidal effects and complement-dependent cytotoxicity of the McAb to EC109 cells was screened by MTT assay.The apoptosis of EC109 cells was detected by flow cytometry with annexin Ⅴ-FITC/PI staining.Morphological change of EC109 was observed by microphotograph.Results:An anti-DR5 monoclonal antibody was obtained.It induced apoptosis of EC109 dose dependently.The cytotoxic action was notably enhanced by addition of complement.The cells growth inhibition ratios reached 83.04%.The apoptotic body and cathepsis were seen in microphotograph.Conclusion:The anti-DR5 monoclonal antibody could induce EC109 cells apoptosis and cause the complement dependent cytotoxic (CDC) effects powerfully.
