Inhibitory effects of antisense oligodexynucleotide on the expression of survivin mRNA and protein in human malignant melanoma cell line A375
- VernacularTitle:反义寡核苷酸抑制人恶性黑素瘤A375细胞survivin mRNA及其蛋白的表达
- Author:
Qinlong LIANG
;
Mei WANG
;
Bing WANG
;
Jiyua ZHAO
- Publication Type:Journal Article
- Keywords:
survivin;
malignant melanoma;
antisense oligonucleotide
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of survivin antisense oligodexynucleotide(ASODN) on the expression of survivin mRNA and its protein in human malignant melanoma cell line A375.Methods Survivin ASODN was synthesized and transfected into melanoma cell line A375 by the liposome-encapsulation.Survivin mRNA and protein were detected by in situ hybridization and immunohistochemistry,respectively. Results At 24h after the transfection,the expression of survivin mRNA was significantly lower in the ASODN group than in the control group(P
