A simple protocol of DNA sequencing with 10% formamide for dissolving G/C compression.
- Author:
Kee Ryeon KANG
1
;
Yeon Woong KIM
Author Information
1. GYEONGSANG NATL UNIV, COLL MED, DEPT BIOCHEM, CHINJU 660280, SOUTH KOREA.
- Publication Type:Original Article
- Keywords:
formamide;
DNA sequencing;
T7 polymerase
- MeSH:
DNA*;
Ice;
Sequence Analysis, DNA*
- From:Experimental & Molecular Medicine
1997;29(4):235-237
- CountryRepublic of Korea
- Language:English
-
Abstract:
Formamide has been widely used in urea/polyacrylamide gel to solve the compression problems that are occasionally found during the DNA sequencing of G/C rich regions. In this study, however, 10% formamide was added in annealing solution in stead of adding to the gel. The compressions were unfolded efficiently with a more rapid annealing reaction on ice in the presence of 10% formamide.
