Experimental study on the cell culture and secretory function of parathyroid cells in rats
- VernacularTitle:大鼠甲状旁腺细胞体外培养及功能的实验研究
- Author:
Xiuqing DUAN
;
Yongning LI
- Publication Type:Journal Article
- Keywords:
Parathyroid gland?Cell culture?Parathyroid hormone
- From:
Chinese Journal of Current Advances in General Surgery
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the culture technique and secretory function of parathyroid cells in rats in order to establish experimental basis of parathyroid cell transplantation.Methods:After parathyroid glands were digested by collagenase and trypsin,isolated parathyroid cells were cultured.The cells cultured for 5 days were observed by transmission electron microscopy.Parathyroid hormone obtained from the cell culture fluid on day 1,3,5 and 7 was measured respectively.Results:Parathyroid cells cultured for 5 days had abundant rough surfaced endoplasmic reticula、Golgi bodies、stab mitochondria and secretory granules.Cultured parathyroid cells could secrete parathyroid hormone normally,especially on the 5th day.Conclusion:Parathyroid cells cultured for 5 days still possess original morphologic structure and have maximum secreting function,so it is an optimal donor for transplantation.
