Three-dimensional reconstruction and visualization of internal microstructure of median nerve
- VernacularTitle:人体正中神经内部显微结构的三维重建与可视化研究
- Author:
Kuo SUN
;
Ping HU
;
Feng ZHANG
- Publication Type:Journal Article
- Keywords:
median nerve;
three-dimensional reconstruction;
three-dimensional visualization;
acetylcholinesterase
- From:
Orthopedic Journal of China
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the application of computer in dealing with two-dimensional image information of human median nerve series freezing tissue sections,in order to develop nerve three-dimensional visualization system(3D Nerve),and finally to reconstruct 3D internal microstructure of human median nerve and realize 3D visualization.[Method]One fresh cadaver median nerve was taken,located with human hair and embedded in OCT.Series freezing tissue sections were made and stained with ACHE histochemistry method,and 2D image information was obtained through high resolution scanner.Microstructure of median nerve was finally reconstructed with 3D Nerve.[Result]Different cross sections of median nerve had quite different number and positions of fasciculi.In addition,characters of fasciculus' s internal nerve fiber were also quite different.Scross sections observation showed that all fasciculi were mixed fasciculi.With the 3D Nerve,the microstructure of median nerve was able to be observed in magnifying visual field at any cross section,and the tracking of stereo course of fasciculi in median nerve became possible.[Conclusion]Reconstructed 3D visualization can reveal the whole microstructure of median nerve and the three dimensional stereo-structure of fasciculi and fasciculus groups exactly and truly.It can provide exact topographic atlas and facilitate precise clixical repair of median nerve injury.
