Adipose tissue derived stem cells transferred with TGF-?_1 gene and differentiated into chondrocytes
- VernacularTitle:TGF-?_1基因修饰诱导脂肪干细胞成软骨分化的实验研究
- Author:
Honglin WANG
;
Fanhong MIN
;
Yanfeng WANG
- Publication Type:Journal Article
- Keywords:
transforming growth factor-?1(TGF-?1);
adipose tissue derived stem cells(ADSCs);
gene transfection;
reverse transcription polymerase chain reaction(RT-PCR);
Western-blot
- From:
Orthopedic Journal of China
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To induce TGF-?1 gene which can increase ECM synthesis into adipose tissue derived stem cells(ADSCs) by employing gene transfer techniques and observe whether TGF-?1 gene could expresse continuously and whether type II collagen and aggrecan are synthesized in order to provide experimental data for constructing tissue-engineering cartilage. [Method]ADSCs were transferred with TGF-?1 gene ,TGF-?1,FN,ColⅡ and aggrecan were detected with RT-PCR and TGF-?1 protein was detected with Western-blot.[Result]RT-PCR demonstrated that the expression of TGF-?1,FN,ColⅡ and aggrecan in the TGF-?1 gene transferred groups increased more evidently than non-gene groups and control groups (P
