Effect of ultra-filtration extract from Danggui Buxue Decoction on spleen cells in H_(22) bearing mouse inducing IL-2 and IFN-?
- VernacularTitle:超滤膜提取当归补血汤对荷瘤小鼠脾细胞IL-2和IFN-?的诱生作用
- Author:
Qingjun LIU
;
Mindi HU
;
Caimei LIU
;
Ting GONG
- Publication Type:Journal Article
- Keywords:
ultra-filter;
Danggui Buxue decoction;
interleukin-2 (IL-2);
interferon-? (IFN-?)
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ultra-filtration extract from Danggui Buxue Decoction (DBD) on immune function of H22 bearing mouse and explore the mechanism through observing the effect of ultra-filtration extract from DBD on the secretion of the cytokines (IL-2,IFN-?).Methods We used membrane separation technique to extract DBD.The content of IL-2 and IFN-? in splenocyte culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA),and the mRNA expression of cytokines (IL-2,IFN-?) in splenocytes was assayed by reverse transcription-polymerase chain reaction ( RT-PCR).Results The content of IL-2 and IFN-? in splenocyte culture supernatant was increased in ultra-filtration extract from DBD groups,significantly in high-dose group (P
