Experimental Study of Multiple-Mouse MRI in Evaluating the Therapeutic Effect of Endostatin for Colorectal Carcinoma in Mice
- VernacularTitle:多鼠MRI评价小鼠结肠癌内皮抑素疗效的实验研究
- Author:
Xin CHENG
;
Lintao QU
;
Shizhuang ZHANG
;
Bin WANG
;
Fanlian MENG
- Publication Type:Journal Article
- Keywords:
colorectal carcinoma;
MRI;
endostatin;
mouse;
subcutaneously implanted tumor model
- From:
Journal of Practical Radiology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of multiple-mouse MRI in evaluating the therapeutic effect of endostatin for transplantation tumor models of colorectal carcinoma in mice.Methods 24 subcutaneously transplantation tumor’s models of colorectal carcinoma (CT-26) in mice were established, 1 week later, 16 tumor-bearing mice were sieved out and divided randomly into two groups: endostatin (ES) group and normal saline (NS) group, treated with intraperitoneal injection of endostatin 6 mg/kg?d 0.2 ml and equal volume of saline respectively for 14 days. Subsequently, MMMRI was performed, and then the mice were killed immediately and the tumors were cut into sections which were stained with hematoxylin and eosin (H&E). Results Subcutaneous fat layer in NS group presented thinner or disappeared on T1WI,while subcutaneous fat layer in ES group presented thicker. The tumors presented inhomogeneous high signal and intratumoral stippled necrosis on T2WI. The tumor’s volumes measured by MRI and pathology were(2723.26?1136.91) mm3 and (3505.76?1350.12) mm3 respectively,there was no difference between these two measures. And there was correlation between MRI results and pathological results. There was no difference of absolute signal intensity between ES group and NS group on T1WI and T2WI. The signal intensity ratio of ES group (3.19?0.28) was higher than which of NS group (2.60?0.47) on T2WI, and there was no difference on T1WI. Conclusion The therapeutic effect for endostatin on transplantation tumor models of colorectal carcinoma in mice can be displayed distinctly in MMMR image, and the inhibition rate of results can be displayed exactly and noninvasively.
