Effect of L-glutamine on liver Bcl-2 mRNA expression after total hepatic inflow occlusion in rats
- VernacularTitle:谷氨酰胺对大鼠肝门阻断后肝脏Bcl-2 mRNA表达的影响及其保护作用
- Author:
Guoping LIU
;
Wenxi ZHU
;
Guangshun YANG
;
Wenping ZHOU
- Publication Type:Journal Article
- Keywords:
Pringle’s manoeuvre?L-glutamine?Apoptosis?Hepatocytes?Genes,Bcl-2
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of L-glutamine(Gln) on liver Bcl-2mRNA expres sion and apoptosis after total hepatic inflow occlusion in rats.Methods:Male Wistar rats were assigned randomly to three groups(n=40):Group A sham-operation group,group B control group,rats were pretreated with 4 ml 0.9% saline intraperitonally twice per day on 5 consecutive days,group C,rats were pretreated with Gln dissolved in 4 ml 0.9% saline intraperitoneally twice per day on 5 consecutive days.The rats from group B and C underwent total hepatic inflow occlusion for 35min by the pringle's manoeuvre.Ten rats from each group were randomly chosen and killed before the initiation of occlusion at 2 h,4 h,24 h after reperfusion respectively.The levels of MDA,GSH in liver tissue were measured.The serum concentrations of ALT,AST,LDH were assayed on a standard biochemistry autoanalyser.The expression levels of Bcl-2 mRNA in liver were assessed by RT-PCR.The apoptosis of liver were observed by DUTP method.The percentage of apoptosis was analyzed.Results:Compared with group B,the levels of GSH in group C increased after reperfusion(P
