Immunohistochemical study on interstitial cells of cajal in Hirschsprung’s disease
- VernacularTitle:Cajal间质细胞在先天性巨结肠分布的研究
- Author:
Ying GAO
;
Zheng QU
;
Lei WANG
;
Xinguo CHEN
;
Linuan GU
- Publication Type:Journal Article
- Keywords:
Hirschsprung’s disease?Interstitial cells of Cajal?Immunohistochemistry
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of interstitial cells of cajal(ICC) in the pathogenesis of HD through observing the distribution of the cells in normal colon and HD's different segments.Methods:The aganglionic、transitional and dilated segments were collected in operations from 25 children with Hirschsprung’s disease.Another 6 normal segmengts were also collected.Each sections was stained by c-Kit immunohistochemistry.The distribution of the interstitial cells of Cajal was observed by light microscope.Results:The ICC in aganglionic segments of colon from Hirchsprung’s disease were decreased significantly and statistically than that in dilated and normal colon.Moreover,whole mount preparations showed that,not only the number of ICC-IM was decreased down,but also the branches of ICC-IM and the cellular network were also broken down.Conclusion:The pathogenesis of HD is related to the abnormal distribution of ICC,which lead to or aggravate the bowel spasm in HD.
