Influence of gonadal hormones on the proliferation of lymphocytes in systemic lupus erythematosus (SLE) patients and the related mechanism
- VernacularTitle:系统性红斑狼疮患者中性激素对淋巴细胞功能影响的研究
- Author:
Xiaoli ZHANG
;
Xiaoyun TANG
- Publication Type:Journal Article
- Keywords:
Systemic erythematosus lupus;
Estradiol;
Testosterone;
Estrogen receptor alpha;
Lymphocytes proliferation
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of gonadal hormones on the function of lymphocytes of SLE patients through detecting the expression of estrogen receptor alpha(ER?) and the function of peripheral blood lymphocytes of SLE patients.Methods:Blood samples of 33 SLE patients and 31 normal controls were collected.Estradiol(E2) and testosterone (T) concentration in peripheral blood were detected by ELISA,the proliferating reaction of T lymphocyte stimulated by ConA were detected by methyl thiazolyl tetrazolium (MTT) assay and the expression of estrogen receptor alpha(ER?)mRNA of peripheral blood mononuclear cells (PBMCs),T lymphocytes and B lymphocytes were detected by RT-PCR.Results:Compared with normal control,the stimulating index of T lymphocyte proliferation decreased(P
