In vitro co-culcure of mouse osteoblasts combined with magnesium alloys
- VernacularTitle:体外镁合金与小鼠成骨细胞联合培养观察
- Author:
Guoning YU
;
Feng PAN
;
Jiuquan WEN
- Publication Type:Journal Article
- Keywords:
magnesium alloy;
osteoblast;
biocompatibility
- From:
Orthopedic Journal of China
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To observe mouse osteoblasts vitality,histomorphology and growing development in vitro co-culture combined with magnesium alloys,confirm the biocompatibility of magnesium alloys and osteoblasts,and try to find out the possibility of magnesium alloys to be a new type of bone surgery implant material.[Method]Mouse osteoblasts were checked by Gomori and Von kossa stained,cultured and developed in vitro.Then the osteoblasts were mixed with magnesium alloys in a cell density of 3?104/ml.After 24、48、72 hours co-culture,the surface of magnesium alloys were observed by scanning electron microscopy(SEM) and confocal microscopy to find the change of osteoblasts.[Result]After culture in vitro,the osteoblasts well developed,and expressed stable character.After co-culture with magnesium alloys,the cells adhered and proliferated on the surface of alloys very well from SEM and confocal microscopy observation,which showed the osteoblasts had great activity and reproduce capability.[Conclusion]Magnesium alloys showed good biocompatibility and bone conduction capability with mouse osteoblasts,so the magnesium alloy is very possible to be a new type of bone surgery implant material.
