Association of CD40 gene 5′UTR C(-1)T,-58038T and C64610G polymorphism with autoimmune thyroid disorders
- VernacularTitle:CD40基因多态性与自身免疫性甲状腺病的相关性
- Author:
Yi ZHANG
;
Benzhang ZHU
;
Yan SUN
- Publication Type:Journal Article
- Keywords:
autoimmune thyroid disorder;
CD40 gene;
single nucleotide polymorphism;
polymerase chain reaction combined with restriction fragment length polymorphism;
amplification refractory mutation system
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate polymorphism of CD40 gene and its relation with autoimmune thyroid disorders(AITD) in China's Northwest region.Methods The study recruited 372 subjects: 165 Graves' disease(GD) and 113 Hashimoto's thyroiditis(HT) and 93 healthy subjects as controls.① Using PCR-restriction fragment length polymorphism(PCR-RFLP) to analyze the C/T polymorphism in the 5′UTR and the-58038T point mutation in exon3.② Using amplification refractory mutation system-PCR(ARMS-PCR) to analyze the C64610G point mutation in exon9.Results ① There were significant differences in allele frequencies of C and genotype CC between GD group and control group in 5′UTR C(-1)T(P
