The effect of aminoguanidine intraperitoneal injection on apoptosis in chondrocyte with osteoarthritis
- VernacularTitle:氨基胍对兔骨关节炎软骨细胞凋亡的影响
- Author:
Yimin YANG
;
Min WANG
;
Meng LI
- Publication Type:Journal Article
- Keywords:
aminoguanidine;
osteoarthritis;
chondrocyte;
apoptosis;
rabbit;
iNOS
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of aminoguanidine by intraperitoneal injection on apoptosis of chondrocyte with osteoarthritis.Methods Totally 32 rabbits were divided into 3 groups at random.Both the experiment and the model groups were fixed by plaster cast in the right hind straighten limb,while the control group received no treatment.The experiment groups were injected with aminoguanidine in abdominal cavity,100mg/(kg?d),and the control groups were injected the same voluminal normal saline in abdominal cavity.At the 4th week,we killed 4 rabbits from the normal group,and 6 from the control group and the experiment group,respectively.At the 8th week,we put the rest to death.We drew the materials from each sample for pathological observation on the whole and under the light microscope by HE and immunohistochemical staining,and counted apoptosis cells by TUNEL for statistical analysis.Results Compared with the control group,the experiment group was more similar to the normal group in general observation and optical microscope observation by HE staining.Both iNOS positive expression cell by immunohistochemical staining and apoptosis cell calculation by TUNEL of the experiment group were fewer than those of the control group(P
