Differential proteomic analysis of Pten~(L/L)MEFs and Pten~(△/△)MEFs cell lines
- VernacularTitle:Pten~(L/L)MEFs与Pten~(△/△)MEFs细胞系的差异表达蛋白质组分析
- Author:
Ruifeng DUAN
;
Gang LI
;
Yingchun HU
;
Dechang WU
;
Yanying HUO
- Publication Type:Journal Article
- Keywords:
PTEN;
two-dimensional electrophoresis(2-DE);
matrix assisted laser desorption/ionization time-of-flight mass spectrometry;
peptide mass fingerprinting
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study protein variation between PtenL/LMEFs and Pten△/△MEFs cells.Methods Two-dimensional electrophoresis(2-DE) was employed to compare the differential expression proteins between PtenL/LMEFs and Pten△/△MEFs cells.Six differential expression proteins were digested in gel by enzyme and the mass of generated peptides was measured by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS).The data obtained from peptide mass fingerprinting(PMF) were searched using the Internet available database and five proteins were identified.Results Compared with that of PtenL/LMEFs,expression level of proteins including phosphoglycerate mutase 1(PGAM1) and peptidyl-prolyl cis-trans isomerase C(PPIC) was up-regulated,whereas expression level of Transgelin 2 was down-regulated in Pten△/△MEFs cells.B and F proteins were both identified to be peroxiredoxin-6.They had similar molecular weight but different PI which might be caused by post-translation modification.B protein was only expressed in Pten△/△MEFs cells.Conclusion The protein profile of Pten△/△MEFs cells displayed obvious difference compared to that of PtenL/LMEFs cells.The results implied that various distinct different proteins might lead to cancer.
