Effect of hydroxycamptothecine on PS_2 and COX-2 of the transplanted tumor in the nude mice by human bile duct carcinoma QBC939 cells
- VernacularTitle:羟基喜树碱对人胆管癌QBC939裸鼠移植瘤PS_2、COX-2的影响
- Author:
Guodong HUANG
;
Yuanhua HUANG
;
Daofu HUANG
;
Meizhen XIAO
;
Lijun TANG
- Publication Type:Journal Article
- Keywords:
bile duct carcinoma;
nude mouse;
transplantation;
QBC939 cell;
PS2;
COX-2
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hydroxycam ptothecine on PS2 and COX-2 in nude mice by human bile duct carcinoma QBC939 cells in order to analyze the correlation between PS2 and COX-2 expressions and the development of the tumor.Methods After the model of the transplanted tumor of subcutaneous tissues located the nude mice was constructed by human bile duct carcinoma QBC939 cells,30 nude mice were randomly divided into 3 groups,namely,control group,hydroxycamptothecine low-dose group and high-dose group.The growth of the transplanted tumor was observed and immunohistochemical staining combined with image analysis was used to determine the expression of PS2 and COX-2 in the tumor tissues.Results Compared with that of the control group,the content of PS2 in the tumor tissues was increased in hydroxycamptothecine low-dose group and high-dose group.The content of COX-2 was decreased and the growth of the transplanted tumor was inhibited in both hydroxycamptothecine low-dose group and high-dose group(P
