Effect of recombinant plasmid pcEgr-p53 stable transfection in combination with X-irradiation on apoptosis and expression of apoptotic proteins in human A549 tumor cells
- VernacularTitle:pcEgr-hp53稳定转染联合X射线诱导人肺腺癌A549细胞凋亡及相关凋亡蛋白表达变化
- Author:
Lihua DONG
;
Yiduo XU
;
Feng LIU
;
Shouliang GONG
- Publication Type:Journal Article
- Keywords:
pEgr-hp53;
X-ray;
A549;
Apoptosis;
Bcl-2;
Bax;
caspase-3
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of recombinant plasmid pcEgr-p53 stable transfection in combination with X-ray irradiation on the apoptotic effect and the changes of Bax,Bcl-2 and caspase-3 protein expressions in human A549 tumor cells.Methods:pEgr-hp53 packaged with liposome was stably transfected into A549 cells in vitro.These cells of expressing A549-hp53 and A549-vect were irradiated with 0,0.5,2 and 5 Gy X-rays,respectively,i.e.8 experimental groups.The A549 cells responding to the apoptotic effect and the changes of Bax,Bcl-2 and caspase-3 protein expressions were measured with TUNEL assay and flow cytometry,respectively.Results:The percentage of apoptotic cells in A549-hp53 plus different dose irradiation group were significantly higher than that in 0 Gy group(P
