Combined effects of different neurotrophins on rat spinal cord neurons
- VernacularTitle:不同营养因子组合对大鼠脊髓神经元的作用研究
- Author:
Wei XIAO
;
Zhuojing LUO
;
Ming HUANG
- Publication Type:Journal Article
- Keywords:
neurotrophin;
spinal cord;
neuron;
neurite outgrowth;
cell culture
- From:
Orthopedic Journal of China
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the combined effects of different neurotrophin couples of NGF,bFGF,BDNF on rat spinal cord neurons.[Method]Spinal cord neurons were obtained from SD rats born within 1d,and then were seeded in culture plates.Different factors and their couples were added in each chamber while DMEM/F12 served as controls,concentration of NGF,bFGF or BDNF were 50ng/ml.Phase contrast microscope observation was done.At the 3rd and 7th day after incubation,the cells were detected by ?-tubulin3 immunofluorescence and Hoechst staining.The length of neuritis was measured,and the numbers of neuron cells and nuclears were determined.At the 1st,3rd,5th,7th and 9th day MTT method was used,and the growth curve was made according to OD results.[Result] The length of axon and positive rate in the experimental groups were superior to those in the control group(P
