The influence of 149 serine on the localization of cdc25b protein in mouse oocytes
- VernacularTitle:149位丝氨酸对cdc25b蛋白在小鼠卵母细胞中定位的影响
- Author:
Hongmei ZHAO
;
Xiaoyan XU
;
Bingzhi YU
- Publication Type:Journal Article
- Keywords:
cdc25b;
Meiosis;
Site mutant;
Subcellular localization
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of 149 serine in the abrogation of cdc25b as a meiosis inducer in mouse oocytes.Methods: Immunofluorescence analysis was used to observe the distribution of cdc25b protein in GV stage and G2/M transition oocytes;Fused plasmids of pEGFP-cdc25b-wt,pEGFP-cdc25b-s149a and vector pEGFP-C3 were microinjected into GV mouse oocytes.After culturing for a certain time,the subcellular localization of protein was observed.Results:cdc25b-wt was distributed mainly in cytoplasm in GV stage oocytes and accumulation of protein was observed during G2/M transition while green fluoresent signal of cdc25b-s149a was observed in nuclear and cytoplasm.Conclusion:The results suggest that there existed a shuffling between nuclear and cytoplasm of cdc25b in GV stage oocytes although they seem to be quilt,the phosphorylation of 149 serine could maintain proper distribution of protein by balancing the speed of protein nuclear export and nuclear import.
