Role of ASC in non-apoptotic programmed cell death of neutrophils induced by ONO-AE-248
- VernacularTitle:ASC在ONO-AE-248诱发的中性粒细胞非凋亡性程序化死亡中的作用
- Author:
Jian YANG
;
Jiajia LIU
;
Xiaoli ZHENG
;
Xiaojie XUE
- Publication Type:Journal Article
- Keywords:
Neutrophils;
ONO-AE-248;
Non-apoptosis programmed cell death;
Apoptosis;
Apoptosis-associated speck-like protein
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the roles of apoptosis-associated speck-like protein containing CARD(ASC),one of differentially expressed proteins in neutrophil death induced by ONO-AE-248.Methods:Human neutrophils were cultured in vitro with or without ONO-AE-248(5?10-5 mol/L).The expression of ASC mRNA was detected by RT-PCR.PMN proteins were extracted at 12 h and then separated by 2D electrophoresis.20 differentially expressed proteins were selected and determined by PDQest 2-DE software.Results:ONO-AE-248 decreased the expression of ASC mRNA strikingly in 2 hours;ASC was one of the important differentially expressed proteins between spontaneous apoptosis and non-apoptosis programmed cell death induced by ONO-AE-248 in 12 hour and ASC protein point was weaker in ONO-AE-248 group.Conclusion:ASC might act as double switch that leads to apoptosis of neutrophils in the high expression and removes the forbiddance of pathway of ONO-AE-248 signals in the low expression,resulting in non-apoptosis programmed cell death of neutrophils.
