Biological differences between healing and normal medial collateral ligament fibroblasts
- VernacularTitle:愈合期与正常内侧副韧带细胞生物学特性差别比较的实验研究
- Author:
Dapeng JIANG
;
Zhaozhu LI
;
Yubo ZHANG
- Publication Type:Journal Article
- Keywords:
knee medial collateral ligament;
fibroblast;
?-smooth muscle actin;
cell proliferation
- From:
Orthopedic Journal of China
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To examine the difference of proliferation and ?-smooth muscle actin(?-SMA) expression between healing and normal medial collateral ligament(MCL) fibroblasts.[Method]Ten adult,male,Sprague-Dawley rats weighing between 350 and 375 g were used in this study.Normal and healing MCL were cut into small pieces in aseptic conditions,and then placed and cultured in culture chamber.Fibroblasts were passaged with 0.25% trypsin.After 24 and 48 hours incubation,MTT was used to measure the cell proliferation.The production of ?-SMA was measured by Western Blot.[Result]After 24 and 48 hours incubation,healing fib roblasts showed absorbency values of 0.49?0.080 and 0.53?0.07 respectively.II was found that healing fibroblasts proliferated faster than normal fibroblasts(P
