Expression of IP-10 and its mRNA in liver biopsy tissue of patients with cirrhosis
- VernacularTitle:肝硬化患者肝活检组织IP-10及其mRNA表达
- Author:
Jian WANG
;
Pingping WANG
;
Guiju XIANG
;
Xiaobin HU
- Publication Type:Journal Article
- Keywords:
Cirrhosis;
IP-10;
mRNA;
PCR;
Immunohistochemistry;
Liver biopsy tissue
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of IP-10 and its mRNA in liver biopsy of patients with cirrhosis.Methods:The typical patients with cirrhosis after HBV infection were selected,and their serum IP-10 concentrations were evaluated with ELISA,the content of IP-10 mRNA in liver biopsy was measured by Real-time PCR.The level of IP-10 mRNA was represented by lg cDNA/lg GAPDH.The expression of IP-10 in liver biopsy tissue was detected by SP immunohistochemistry stain.Results:The levels of IP-10 in serum and its mRNA in liver biopsy of patients with cirrhosis were (299.3?77.2)pg/ml and (0.856 3?0.150 6),lg cDNA/lg GAPDH respectively,which were significantly higher than those of control groups(P
