The effect of LPS and IL-13 on IL-12 production of mesangial cells in vitro
- VernacularTitle:LPS和IL-13影响系膜细胞表达IL-12的研究
- Author:
Yuan WU
;
Liming JIANG
;
Xiaowen CHEN
;
Huijuan HE
- Publication Type:Journal Article
- Keywords:
LPS;
Interleukin-13;
Interleukin-12;
Mesangial cells
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the possible role of LPS and IL-13 on IL-12 production of mesangial cells in vitro.Methods:The production of IL-12 in mesangial cells was detected with ELISA kit under following different treatments:①Mesangial cells were cultured with LPS(20 ?g/ml)under different time;②The cells were cultured with different concentrations of LPS for 24 h.For observation of the effect of IL-13 on IL-12 production by mesangial cells,three groups were designed:control group,LPS(10 ?g/ml)group and different concentrations of IL-13 with LPS (10 ?g/ml).ELISA was applied to detect IL-12 in supernatants fluid from mesangial cells cultured for 24 h.The expression of IL-12p40 mRNA by mesangial cells cultured for 20 h was evaluated by RT-PCR.Results:Under basal conditions (no LPS) the production of IL-12 was hardly detected.The levels of IL-12 stimulated by LPS was significantly increased in certain doses,but the levels of IL-12 reduced accompanied with the doses and time increasing of LPS stimulantion.IL-13(1-100 ng/ml) inhibited the protein and mRNA expression of IL-12 in a dose-dependent manner(P
