Effects of 2,5-hexanedione on Protein Kinase Contents in Rats Spinal Cord
- VernacularTitle:2,5-己二酮对大鼠脊髓组织蛋白激酶含量的影响
- Author:
Qingshan WANG
;
Liyan HOU
;
Cuili ZHANG
- Publication Type:Journal Article
- Keywords:
Environmental pollutants;
2,5-hexanedione;
Toxic neuropathy;
Protein kinase;
Phosphorylation
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the role of protein kinase A (PKA),protein kinase C (PKC) and cyclin dependent kinase5 (CDK5) in the alterations of neurofialments (NFs) in 2,5-hexanedione (HD) induced toxic peripheral neuropathies. Methods HD was administrated to male Wistar rats by intraperitoneal injection at doses of 200 or 400 mg/kg,once a day,for 8 consecutive weeks. The relative contents of PKA,PKC,p35 precursor and CDK5 in spinal cord were determined by using SDS-PAGE and Western Blotting. Results In the cytosolic fraction of spinal cord,the levels of PKA and PKC in 400 mg/kg group significantly increased (P
