Genetic Toxicity of Europium Nitrate to Vicia faba Root Tip Cells
- VernacularTitle:硝酸铕对蚕豆根尖细胞遗传毒性的研究
- Author:
Ling SUN
;
Huifang ZHANG
;
Ruiqing YANG
- Publication Type:Journal Article
- Keywords:
Rare earth;
Europium;
Vicia faba L;
Micronucleus tests;
Mitotic index;
Chromosome aberrations
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the genotoxicity of europium nitrate to the Vicia faba L root-tip cells. Methods The primary roots of Vicia faba L were cultured for 6 hours in the solution of Eu series(64,32,16,8,4,2,1 ?g/ml) at 25℃.And then the roots were repair-cultured in double distilled water for 24 h. The root tips of Vicia faba L were dissected and fixed,stained and the micronucleus rate,mitotic index and the rate of chromosomal aberrations were determined. Results From 1 ?g/ml to 32 ?g/ml,the rate of micronucleus increased with the increasing of the europium nitrate dosage(D),presented a dosage-effect relationship(r=0.949). From 2 ?g/ml to 64 ?g/ml,mitotic index decreased with the increasing of the europium nitrate dosage,presented a dosage-effect relationship(r=-0.852). From 1 ?g/ml to 64 ?g/ml,chromosome fragments(CF) and frequency of chromosomal aberrations(FCA) increased with the increasing of the europium nitrate dosage with a dosage-effect relationship significantly(rD-CF=0.915,rD-FCA=0.872). Conclusion Europium nitrate has the certain genetic toxicity to the Vicia faba L root-tip cells. The chromosome fragments may be one of the reasons to produce micronucleus.
