Expression of Caspase-3 in Renal Glomerulus Cells in Rats with Sub-chronic Arsenic Poisoning
- VernacularTitle:亚慢性砷染毒对大鼠肾小球细胞半胱氨酸蛋白酶-3表达的影响
- Author:
Yuanhui LI
;
Chaosheng KANG
;
Na LI
- Publication Type:Journal Article
- Keywords:
Arsenic poisoning;
Caspase-3;
Apoptosis;
Glomerulus cells
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of caspase-3 on the apoptosis of glomerulus cells in chronic arsenic poisoning. Methods Sprague-Dawley(SD) rats in cleanliness grade were randomly divided into high-dose group,low-dose group and control group,10 in each (5 males and 5 females). The rats in high and low groups were treated with As2O3 through drinking water,10 and 0.4 mg/(kg?d) respectively,for four months. The content of arsenic in the blood and urine was determined. The expression of caspase-3 in the glomerulus cells was detected by SABC immunohistochemistry and analyzed through image patterns. Results Compared with the control group,a higher arsenic content in the blood and urine,more positive cells of caspase-3 and lower OD value the glomerulus cells were found in both of high-dose and low-dose groups. Compared with low-dose group,a higher arsenic content in the blood and urine,more positive cells of caspase-3 and the lower value of grey degree in the renal glomerulus cells were all found in high-dose group. Conclusion The obvious increase of caspase-3 in the glomerulus cells may play a role in the apoptosis of the glomerulus cells induced by chronic arsenic poisoning.
