Experiment study of the repairing of cartilage defects using cells with bioceramic scaffold
- VernacularTitle:细胞复合?-磷酸三钙生物陶瓷修复软骨缺损的实验研究
- Author:
Lei ZHANG
;
Shui SUN
;
Qiang REN
- Publication Type:Journal Article
- Keywords:
tissue engineering;
bioceramic;
repair of cartilage;
chondrocyte;
osteoblast
- From:
Orthopedic Journal of China
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To evaluate the feasibility of repairing cartilage defect with tissue-engineered cartilage ,which was made of chondrocytes and osteoblast seeded onto a biodegradable porous bioceramic ?-tricalcium phosphate (?-TCP).[Method] A porous bioceramic template of ?-TCP was created in the shape of a circular cylinder. Chondrocytes and osteoblasts induced from dog bone marrow stem cells were seeded onto the ?-TCP and then kept them growing together for 1 week prior to transplantation into the cartilage defects. After 12 , 16 weeks ,the specimens were harvested and examined macroscopically, histologically and immunohistochemically. [Result] Gross morphological and histological analysis of the specimens from the ?-TCP complex demonstrated new cartilage construction. The result was better than that in control group. [Conclusion] These findings suggest that porous bioceramic (?-TCP) is a good "matrix" for chondrocytes and osteoblasts, and can be used for cartilage engineering.
