Expression and significance of toll-like receptor 4 by peripheral blood mononuclear cell in rats with adjuvant-induced arthritis
- VernacularTitle:Toll样受体4在佐剂性关节炎模型大鼠外周血单核细胞中的表达及意义
- Author:
Dianzeng ZHANG
;
Liesu MENG
;
Hongying WANG
- Publication Type:Journal Article
- Keywords:
adjuvant-induced arthritis;
rat;
toll-like receptor 4;
peripheral blood monocyte
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To demonstrate the role of innate immunity reaction in rheumatoid arthritis and other autoimmune diseases by studying the expression of toll-like receptor 4(TLR4) by peripheral blood mononuclear cell(PBMC) in rats with adjuvant-induced arthritis(AIA) and analyzing the relationship between expression of TLR4 by PBMC and score of arthritis severity.Methods Both the proportion of CD14+TLR4+ cells in CD14+ cells and the mean intensity of fluorescence(MIF) of TLR4+ cells were analyzed by flow cytometry.Expression of TLR4 mRNA by PBMC was determined by Northern blot.CD14+TLR4+/CD14+ ratio,TLR4 MIF and expression of TLR4 mRNA by PBMC in the model group at 15 days after AIA were compared,respectively,with normal control group and model group at 25 days.The correlation between TLR4 MIF and score of arthritis severity was analyzed by Mann-Whitey U test.Results CD14+TLR4+/CD14+ ratio,TLR4 MIF and TLR4 mRNA expression in model group at 15 days after AIA were obviously increased compared with those in control and model groups at 25 days(P0.05).It indicated that TLR4 participated in the pathogenesis of rheumatoid arthritis.Conclusion TLR4 expressed by PBMC is closely associated with pathogenesis and pathophysiology of rheumatoid arthritis,which suggests that innate immunity response plays an important role in pathogenesis of RA and other autoimmune diseases.
