Fragile histidine triad transcription abnormalities and human papillomavirus16 infection in human cervical carcinoma
- VernacularTitle:人宫颈癌FHIT基因的表达改变与HPV16感染的关系
- Author:
Jin HAN
;
Wei CHEN
;
Xu LI
- Publication Type:Journal Article
- Keywords:
cervical cancer;
fragile histidine triad(FHIT);
deletion;
human papillomavirus 16(HPV16)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relationship between fragile histidine triad(FHIT) transcription abnormalities and HPV16 infection and human cervical tumorigenesis.Methods Total RNA from 5 cervical carcinoma cell lines(SiHa,HeLa,RJC-1,CS1213 and C4-1),58 primary cervical cancer specimens and 18 normal cervical epithelial tissues were extracted and FHIT transcripts were characterized by a two-step(nested) reverse transcription(RT)-PCR.The seven of the PCR products with different size were purified and sequenced.HPV16 infection was assessed by PCR.Results ① There were altered FHIT transcripts in SiHa,HeLa and C4-1 cells.Aberrant FHIT transcripts were detected in 39 out of the 58 cervical cancer samples(67.2%),but none out of 18 in the normal cervix tissue specimens(0%);HPV16 infections were identified in 37 of the 58 cervical cancer tissues(63.8%),but 1 in the 18 normal cervical epithelial tissues(5%),which showed a significant difference between these two groups(P0.05).③ The exon 5 and exon 6 were mainly deleted in the altered FHIT transcripts and no insertion and point mutation were found by DNA sequencing.Conclusion Aberrant FHIT expression was significantly common in cervical cancers and was correlated with HPV16 infection.These findings suggest that the tumor suppressor gene FHIT and high risk HPV16 may play a very important role in human cervical carcinogenesis.
