Experimental study on the preparation and cytotoxicity of a rhBMP-2 loaded amorphous calcium phosphate delayed release nano-sized material
- VernacularTitle:无定形磷酸钙负载rhBMP-2纳米缓释体的制备及细胞毒性实验
- Author:
Haoyu WANG
;
Xiaotao WU
;
Shaodong ZHANG
- Publication Type:Journal Article
- Keywords:
rhBMP-2;
amorphous calcium phosphate;
cell culture;
BMSCs;
cytotoxicity
- From:
Orthopedic Journal of China
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objeetive] To develop the recombinant human bone morphogenetic protein-2(rhBMP-2)loaded amorphous calcium phosphate(ACP)delayed release nano-sized material,investigate its cytotoxicity of cell,and provide a reference for the experiment of composite material in vivo.[Method]The rhBMP-2/ACP delayed release nano-sized material were prepared by chemical wet method and cultured on rabbit bone marrow-derived mesenchymal stem cells(BMSCs)in vitro.Then the adhesion,proliferation,growth and functional expression of BMSCs were measured.[Result]Cytotoxicity test demonstrated that rhBMP-2/ACP delayed release nano-sized material had not affect the percentage of cell's proliferation with material-extracted liquid cultured with BMSCs and the cytotoxicity was graded zero.The adhesion,proliferation,configuration of the cells on the surface of this material were identical to the control group.[Conclusion]It was suggested that rhBMP-2/ACP delayed release nano-sized material might have good cellular biocompatibility,no cytotoxicity and not effected the normal functional expression of BMSCs in vitro.
