Effect of Leptin on the apoptosis of thymocytes induced by LPS
- VernacularTitle:瘦素对LPS诱导的小鼠胸腺细胞凋亡的保护作用
- Author:
Xiaojuan REN
;
Guiqin WANG
;
Guangtao YAN
;
Xiaohui WANG
;
Ji LIN
;
Zihui DENG
- Publication Type:Journal Article
- Keywords:
Leptin;
LPS;
Thymocytes;
caspase3
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Leptin on the apoptosis of thymocytes induced by lipopolysaccharide(LPS) in mice.Methods:The toxicity of LPS on thymocytes was evaluated using MTT colorimetric assay.Thymocytes apoptosis was detected using Annexin V-FITC/PI double staining flow cytometry.caspase3 mRNA were measured by RT-PCR.Results:After cultured for 6 h,apoptosis of thymocytes was identified by flow cytometry.Apoptosis rate in LPS group (55.12?0.656)% was significantly higher than that in control group (9.58?0.605)% (P
