Research on combinative effect of methotrexate and caffeine on osteosarcoma cell line
- VernacularTitle:氨甲喋呤联合咖啡因对骨肉瘤细胞株的作用观察
- Author:
Gang SUN
;
Shuzhong LI
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
MTX;
caffeine;
tumor cell,incubation
- From:
Orthopedic Journal of China
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To observe whether or not caffeine can improve the cyto-toxicity effect of methotrexate(MTX) on osteosarcoma cell line.[Method]Osteosarcoma cell(OS-732)were incubated with no drug,caffeine,MTX or MTX adding caffeine separately,and were named as control group,caffeine group,MTX group and mixing group respectively.The ratios of cells on G2M phase of each group were measured with flow cytometry after 48 h incubation and the cell inhibition ratios were measured with the MTT colorimetric analysis after 72 h incubation.[Result]The ratios(%)of cells on G2M phase were control group(23.210?0.416),caffeine group(23.120?0.440),MTX group(28.770?0.531)and mixing group(0.105?0.002)(P
