The Mechanisms of Repression of Testosterone Biosynthesis in Rats Exposed to Dibutyl Phthalate
- VernacularTitle:邻苯二甲酸二丁酯抑制大鼠睾酮合成的影响机制
- Author:
Xiaofeng ZHANG
;
Zi LI
;
Jing ZHENG
- Publication Type:Journal Article
- Keywords:
Dibutyl phthalate;
Testosterone;
Corticosterone;
Luteinizing hormone
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of repression of testosterone biosynthesis in rats exposed to dibutyl phthalate (DBP). Methods Immature male rats were randomly divided into 5 groups involving 4 experimental groups and one control group. Animals were administered orally either corn oil only (vehicle control) or DBP (0.25, 0.50, 1.00, 2.00 g/kg) for 30 days. After 30 days exposure, the half of animals were sacrificed with testis, epididymis and adrenal selected and weighed. The ratio of organ to body weight were calculated and histopathological examination were examined. Testosterone (T), luteinizing hormone (LH), 17?-estradiol (17?-E2) and corticosterone (CORT) were detected by radioimmunoassay. After 15 days, the rest of animals were sacrificed and above-mentioned indexes were measured. Results The ratio of testis and epididymis to body weight reduced significantly at doses of 0.50 g/kg DBP and above. The testicle atrophy was observed, germinal epithelium became thin and the amount of reproductive cells obviously decreased. All of those were not repaired in convalescence stage. In above 1.00 g/kg DBP groups, the level of T, LH and 17?-E2 decreased (P
