Rescue role of Bu-Shen-Yi-Qi Formula for trophoblast apoptosis induced by TNF-?/IFN-?
- VernacularTitle:补肾益气方对TNF-?/IFN-?诱导的早孕期人滋养层细胞凋亡的影响
- Author:
Haiyan WANG
;
Suiqi GUI
;
Qindi ZOU
;
Xuelian LI
- Publication Type:Journal Article
- Keywords:
Bu-Shen-Yi-Qi Formula;
Drug-containing serum;
First trimester trophoblasts;
TNF-?;
IFN-?;
Apoptosis
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the rescue role of Bu-Shen-Yi-Qi Formula on the apoptosis of first trimester trophoblasts induced by TNF-?/IFN-?.Methods:Cell viability was measured by MTT assay,and cell numbers in sub-G1 phase were determined by FCM.The amount of apoptotic cells was evaluated by TUNEL,caspase-3 enzyme activity was examined by caspase-3 active assay kit.Bcl-2 and XIAP protein expression were detected by Western blot.Results:(1)Cell viability decreased,but apoptotic cells increased and the cells in sub-G1 phase accumulated when cultured with TNF-? and IFN-? for a 24 h and the reaction became more serious following a 48 h culture.However,cells cocultured with drug-containg serum showed enhanced cell viability,less apoptotic cells and low enzyme activity.Also,cells in sub-G1 phase decreased and Bcl-2,XIAP protein expression were enhanced when the drug-containg serum were added.This change showed time and dosage dependent.Cell viability cultured with blank serum had no significant changes.Conclusion:Bu-Shen-Yi-Qi Formula rescue trophoblasts apoptosis induced by TNF-? and IFN-?.
