Effects of Selenium on Endothelial Cell Growth Cultured in vitro
- VernacularTitle:硒对体外培养血管内皮细胞的影响
- Author:
Xiaodong HOU
;
Xinying LIN
;
Jianchao BIAN
- Publication Type:Journal Article
- Keywords:
Selenium;
Human umbilical vein endothelial cells;
Cell culture
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the growth of human umbilical vein endothelial cell(HUECV) cultured with different dose of selenium. Methods HUVEC were cultured with selenium in various concentration (50、100、500、1000、2000 nmol/L) for 48 h in vitro. The morphological changes of cells were observed, activity of cell was measured by MTT method, and at the same time the levels of SOD, GSH-Px, MDA, NO, iNOS, eNOS in the culture were determined. Results At the doses of 1 000-2 000 nmol/L, the cells were damaged and revealed related morphological change. Cell activity was significantly higher at the dose of 100 nmol/L compared with that of control (P
