Effects of pCD-Em10 on immune response in mice infected with alveolar hydatid cyst of Echinococcus multilocularis
- VernacularTitle:pCD-Em10对泡球蚴感染小鼠免疫应答的影响
- Author:
Wengui LI
;
Youming ZHU
;
Hong WANG
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
pCD-Em10;
Immune response
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of pCD-Em10 on immune responses in mice infected with alveolar hydatid cyst of Echinococcus multilocularis(Em).Methods:BALB/c mice were intramuscularly vaccinated with pCD-Em10 and then challenged with Em protoscoleces at the 8 w after vaccination.The mice were killed in 18th week of infection to count the rate of reduced alveolar hydatid cyst weight;The spleens were gathered to separate splenocytes,and the subsets of CD4+and CD8+T cells were measured by FACsort.The splenocytes were cultured by stimulation with EmAg or ConA,their supernatants were gathered to measure level of IL-2,IFN-?,TNF-? and IL-4 by ELISA Kits.Apoptotic rate of splenocytes was measured by FACsort.BCG or PBS injection served as control.Results:In the group of PCD-Em10 immunization,the rate of reduced alveolar hydatid cyst weight was 71.17%,CD4+ subset increased obviously,and the levels of IFN-? and TNF-? increased remarkably,but that of IL-4 decreased.Apoptotic rate in the pCD-Em10 group was lower than that in the PBS control.Conclusion:pCD-Em10 may induce a protective immune response in mice infected with alveolar hydatid cyst of Echinococcus multilocularis.
