Study on modulation for immunosupperssion by WWOX in Lewis lung cancer cells
- VernacularTitle:WWOX基因调控Lewis肺癌细胞免疫抑制效应研究
- Author:
Wei YANG
;
Hongyan YUAN
;
Yaping CHANG
- Publication Type:Journal Article
- Keywords:
WWOX;
Gene regulation;
Lewis lung cancer;
Immunosuppression
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of WWOX regulating immunosuppression in Lewis lung cancer cells.Methods:Transfected pCDNA4.0/myc-WWOX plasmid was transfected into Lewis lung cancer cells to detected the effect of cell supernatant on generation of lymphocytes by MTT method,and mRNA expression of immunosuppression factors by semi-quantitative RT-PCR.Function change of NK,CTL to kill tumor and lymphocte proliferation were analyzed by MTT and tumor volume.Results:The suppression of the supernatant to generation of lymphocytes was present compared with control group(P
