Relationship between high mobility group box-1 protein expression and gut mucosal barrier dysfunction during severe acute pancreatitis
- VernacularTitle:重症急性胰腺炎大鼠HMGB1表达与肠黏膜屏障损害的关系
- Author:
Zhenggang LUAN
;
Cheng ZHANG
;
Chunlin GE
;
Xiaochun MA
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis?Intestinal mucosal barrier?High mobility group box-1 protein
- From:
Chinese Journal of Current Advances in General Surgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between high mobility group box-1 protein (HMGB1) expression and gut mucosal barrier dysfunction during murine severe acute pancreatitis (SAP). Methods:Forty-eight male health adult Wistar rats were divided randomly into Control group and SAP groups. The concentration of plasma D-lactate and the activity of myeloperoxidase (MPO) in the intestinal tissue were determined. The expression of HMGB1 mRNA in intestinal mucosa was detected by reverse transcription polymerase chain reaction (RT-PCR) and the activity of HMGB1 was determined by Western blot. Results:Plasma D-lactate and MPO reached a peak level at 24h (16.41?4.65)?g/mL for Plasma D-lactate and(26.76?3.63)U/g for MPO respectively, (P
