Determination of Nicotine in Tobacco by High Performance Liquid Chromatography
- VernacularTitle:烟草替代产品中尼古丁的高效液相色谱测定法
- Author:
Changming DING
;
Shaobin LIN
- Publication Type:Journal Article
- Keywords:
Smoke pollution;
Nicotine;
Chromatography, liquid
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a high performance liquid chromatography (HPLC) method for the determination of nicotine in tobacco. Methods The nicotine was separated completely by C18 column (column temperature∶35℃ ), and was determined by PDA detector at wave length of 260 nm. Mobile phase was acetonitrile: 0.08 mmol/L KH2PO4 (V∶V= 1∶9) at the flow rate of 1.0 ml/min. Results The detection limits is 0.40 ?g/ml. The linear range was 0.00-60.0 ?g/ml. The recovery rates were 97.8%-103% and the relative standard deviations was 2.29% (n=5). Conclusion The method was simple, rapid, sensitive and accurate for the determination of nicotine.
