Prepartion and immunohistochemical study of monoclonal antibodies to duck thymocyte
- VernacularTitle:抗鸭胸腺细胞单克隆抗体的制备及其免疫组织化学研究
- Author:
Hongtao LI
;
Bo MA
;
Junwei WANG
- Publication Type:Journal Article
- Keywords:
Duck;
Thymocyte;
mAb;
T lymphocyte;
Dendritic cell
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the duck thymocyte surface antigens.Methods:Repeated immunization of normal BALB/c mice with separated thymocyte from mature duck result in the production of mAbs which react with duck thymocyte surface antigens.Applying the immunohistochemistry assay,tracing the originating and distributing of lymphocytes which reacted with mAb in spleen,thymus,and bursa of duck different development stages.Results:Five monoclonal antibody-producing hybridomas(B6,B8,C12,E6,H5)were isolated.According to the result of immunohistochemistry assay,there were distributing strained lymphocytes in thymus,bursa and spleen of duck.The stained cells belonged to different populations.Conclusion:The mAb C12 reacted with the common cell surface marker of between mature T lymphocytes and dendritic cells.
