Role of mesenchymal stem cells in rat allogeneic bone marrow transplantation
- VernacularTitle:骨髓间充质干细胞对大鼠异基因骨髓移植后移植物抗宿主病的影响
- Author:
Baoen SHAN
;
Congrong YANG
- Publication Type:Journal Article
- Keywords:
MSCs;
Allo-BMT;
GVHD
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The effect of rat mesenchymal stem cells(MSCs)on graft versus host disease after allogeneic co-transplantation with bone marrow in rat was investigated.Methods:MSCs from bone marrow cells of rat were isolated and cultured in vitro.The purity of MSCs was identified with the spindle-fibroblastic morphological characterization and the phenotypes were analyzed with flow cytometry.The suppressive effect of MSCs on T cell proliferation was analyzed using mixed lymphocyte cultures(MLR).Allogeneic BMT model from SD rats to Wistar rats was established,and then,MSCs were co-transplanted with bone marrow cells at different ratio,the suppressive effect of MSCs on GVHD was studied by clinical symptom,Kaplan-Meier survival curve,and histological analysis.Results:In vitro,when MSC were added to the mixed lymphocyte cultures,the proliferation of T cells were inhibited markedly in a dose-dependent manner(P
