Protection of calcitonin on articular cartilage of rabbit knee joint with osteoarthritis in vivo and in vitro
- VernacularTitle:降钙素在体内和体外实验中对兔关节炎关节软骨的保护作用
- Author:
Nan ZHANG
;
Liu ZHANG
;
Huan ZHENG
- Publication Type:Journal Article
- Keywords:
calcitonin;
osteoarthritis;
matrix metalloproteinase-3;
chondrocyte
- From:
Orthopedic Journal of China
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objeetive To access whether calcitonin exerts a chondroprotective action in unstable knees which induce experiment anterior cruciate ligament transaction(ACLT)on the right knee and were divided randomly into two groups-ACLT group and ACLT+CT group.ACLT+CT group began to receive a daily subcutaneous injection of salmon calcitonin at a does of 5 IU per kg of body weight for 6 weeks;in contrast,ACLT group received NS at the same does.All rabbits were killed on day 49 postoperation cartilage sections were stained with safranin-O-fast green,HE and immunostained MMP-3 antibodies.Chondrocytes expressions of MMP-3 and aggrecan were further examined by Realtime RT-PCR. Result The score,the grey value of Safranin-O-fast green of ACLT+CT group were higher than ACLT group(P
