Cloning and expression of hepatitis C core protein gene
- VernacularTitle:丙型肝炎病毒核心蛋白基因的克隆及表达
- Author:
Ju SUN
;
Yonglie CHU
;
Fengliang JIANG
;
Xiaohong JING
;
Changbin CHAI
;
Yingxin XIE
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
core protein;
pBV220 vector;
gene expression
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the fragment of hepatitis C virus(HCV) core gene and express it in E.coli.Methods The fragment of HCV core gene(approximate 366bp) was amplified by PCR and inserted into the pMD18-T vector.The cloned HCV core gene,which was confirmed by the digestion with EcoRⅠ/BamHⅠ,was subcloned into the expression vector pBV220 to construct recombinant plasmid pBV220/HCV-C.The expressed gene product was identified by SDS-PAGE and Western blotting.Results The fragment of HCV core gene was expressed successfully after temperature induction and a protein of 14 000 u was resulted.Conclusion Expression of the HCV-C gene in E.coli was achieved,which may be helpful for further studies on characterizations of HCV-C gene.
