THE IN VITRO INHIBITION OF CEREBRAL GABA DEGRADATION BY THE LIVER HOMOGENATE SUPERNATANT OF MICE
- VernacularTitle:小鼠肝匀浆对脑GABA降解的抑制作用
- Author:
Tongchaun HE
;
Zhengtai WANG
- Publication Type:Journal Article
- Keywords:
GABA Degradation;
Inhibiton of GABA Degradation;
GABA-T;
Hepatic Encephalopathy
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The cerebral GABA-T activity of mice was determined by the modified coupled-enzyme spectroflourimctry, and the method was proved stable and feasible. The cerebral GABA degradation of mice in vitro was inhibited by the sera from the mice with hepatic coma induced by galactosamine. The supernatant of liver homogenate of both control and hepatic coma groups can inhibit the cerebral GABA degradation too. The inhibitory capacity is proportional to the volume of LHS added in certain conditions, and reaches a maximum inhibitory rate of 80%. The inhibition can not be relieved by pyridoxal phosphate.The inhibitory effector of cerebral GABA degradation has been regarded as liver specific or mainly presented in the liver since no appreciable inhibition was found after the addition of the supernaiants of kidney, heart, lung and spleen homogenates. The possible contribution of GABA degradation inhibition by LHS to the pathogenesis of hepatic encephalopathy has also been discussed
