Oligonucleotide sensitizes A375 cells to cisplatin
- VernacularTitle:Survivin反义寡核苷酸增加A375细胞对顺铂的敏感性
- Author:
Mei WANG
;
Xiaoli WANG
;
Bing WANG
;
Shengshun TAN
- Publication Type:Journal Article
- Keywords:
survivin;
cisplatin;
malignant melanoma;
cell apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether targeting survivin has the potential to sensitize A375 cells to chemotherapy.Methods By trypan blue exclusion assays we observed A375 cell proliferation inhibition induced by survivin antisense oligonucleotide(ASODN) and cisplatin.Results A375 cells treated with a combination of 5 ?mol/L ASODN and 0.5 mg/L cisplatin,and approximately 61.3% of the cells showed signs of cell death 72 h after the start of transfection trypan blue exclusion assays.Compared to treatments with either oligonucleotide or cisplatin alone,there were significant differences among the groups(P
